|
KMID : 0644120090220040028
|
|
Journal of Oriental Gynecology
2009 Volume.22 No. 4 p.28 ~ p.45
|
|
|
The Effect of Yukmijihwangtanghapyijihwangagambang on Melanin Synthesis and Related Gene Expressions in B16F10 Mouse Melanoma Cell
|
|
Shin Sun-Mi
Yoo Dong-Youl
|
|
|
Abstract
|
|
|
Purpose: This study was performed to elucidate the inhibitory effect of Yukmijihwangtanghapyijihwangagambang (YM) on melanin synthesis in B16F10 melanoma cells.
Methods: To demonstrate the inhibitory effects of YM on melanin synthesis, we measured the amount of released and produced melanin in B16F10 melanoma call. Also, we evaluated tyrosinase-activity in vitro as well as in B16F10 melanoma call. And to investigate the action mechanism, we assessed the gene expression of tyrosinase, TRP-1, TRP-2, PKA, PKCβ, ERK-1 ERK-2, AKT-1 and MITF in B16F10 melanoma call.
Results: 1. YM decreased the release and production of melanin in B16F10 melanoma cells. 2. YM decreased tyrosinase activity in vitro and in B16F10 melanoma cells. 3. YM decreased the expression of tyrosinase, TRP-1, TRP-2 in B16F10 melanoma cells. 4. YM decreased the expression of PKA, PKCβ in B16F10 melanoma cells. 5. YMincreased the expression of ERK-1, ERK-2 and AKT-1 in B16F10 melanoma cells. 6. YM decreased the expression of MITF in B16F10 melanoma cells.
Conclusion: From these results, it may be concluded that YM has antimelanogenetic effects.
|
|
|
KEYWORD
|
|
|
Yukmijiwhangtanghapyijihwangagambang (YM), Melanin synthesis, TRP, PKA, PKCβ, ERK, AKT, MITF
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|